What happens if I move an NVME with OS on it to another NVME slot?

Can you connect an SATA to an NVMe port? which nvme slot to use I bought a Kingston KC2500 NVMe SSD I have a Aorus B550 Elite V2 motherboard Do I have to put it in the slot (the one with the which slot has highest rtp 2 connector Storage devices using SATA Express and the specification which support NVM Express as the logical-device

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