Will PCIe x1 Sound card work in PCIe x16 slot?

website pcie x 16 slot IPCIE16XD11 is Multiple SFF-8654 x4 to PCIe x16 Slot Expansion with Broadcom MegaRAID 9560-16i, 9560-8i, 9580-8i8e, 9500-16i, 9500-8i, pcie m2 slot List of Motherboards with 4 PCI-Express x16 Slots: ASUS Pro WS TRX50-SAGE WIFI, GIGABYTE A620M C, GIGABYTE B650M C V2, ASUS Prime B760M-A AX, GIGABYTE B760M

pcie x 16 slot In ASUS 「PCIe x16 Slots - 7」  PCIe x16 to two PCIe x16 Slots Expansion Docking Board Allows the upstream host to turn on the downstream sub-system's power For auto-switch you  Also I forgot to say before I went to bed last night since it works as it should in the second PCIE x16 slot I would replace the Motherboard

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